Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
نویسندگان
چکیده
منابع مشابه
Real time measurements of membrane protein:receptor interactions using Surface Plasmon Resonance (SPR).
Protein-protein interactions are pivotal to most, if not all, physiological processes, and understanding the nature of such interactions is a central step in biological research. Surface Plasmon Resonance (SPR) is a sensitive detection technique for label-free study of bio-molecular interactions in real time. In a typical SPR experiment, one component (usually a protein, termed 'ligand') is imm...
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Surface plasmon resonance (SPR) has become one of the most important techniques for studying macromolecular interactions. The most obvious advantages of SPR over other techniques are: direct and rapid determination of association and dissociation rates of binding process, no need for labelling of protein or lipids, and small amounts of sample used in the assay (often nM concentrations of protei...
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Nanophotonic technique has been attracting much attention in applications of nano-bio-chemical sensing and energy conversion of solar energy harvesting and enhanced energy transfer. One approach for nano-bio-chemical sensing is surface plasmon resonance (SPR) imaging, which can detect the material properties, such as density, ion concentration, temperature, and effective refractive index in hig...
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The application of surface plasmon resonance (SPR) measurements to the study of ultrathin organic and inorganic films adsorbed onto gold surfaces utilizing nearinfrared (NIR) excitation from 800 to 1152 nm is described. SPR scanning angle measurements of film thickness are demonstrated at 814 and 1152 nm using lowpower diode and HeNe laser sources, respectively. Several advantages of SPR in the...
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The application of surface plasmon resonance (SPR) measurements to the study of ultrathin organic films adsorbed onto gold surfaces utilizing near-infrared (NIR) excitation from a Fourier transform (FT) spectrometer is described. The FT-SPR experiment measures the NIR reflectivity spectrum from a prism/gold film/water assembly at a fixed angle of incidence approximately 1-2° greater than the cr...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/51937